Amnis ImageStreamx System - Downloads

Product Literature | Application Notes | Selected Publications | Posters

Product Literature

ImageStream Brochure (Adobe PDF, 2.0 MB)
Extended Depth of Field Brochure (Adobe PDF, 858 KB)
Frequently Asked Questions (Adobe PDF, 771 KB)

Application Notes

09-008 γ-H2AX spot counting 2009 (Adobe PDF, 373 KB)
09-007 Multiplexing 2009 (Adobe PDF, 661 KB)
09-006 Shape Change 2009 (Adobe PDF, 725 KB)
09-005 FRET 2009 (Adobe PDF, 462 KB)
09-004 Stem Cell 2009 (Adobe PDF, 597 KB)
09-003 Phagocytosis 2009 (Adobe PDF, 776 KB)
09-002 Bacteria 2009 (Adobe PDF, 387 KB)
09-001 Oceanography 2009 (Adobe PDF,983 KB)
08-001 Internalization & Sub-cell Organelle Trafficking 2008 (Adobe PDF, 1.78 MB)
08-002 Immune Synapse, ADAP 2008 (Adobe PDF, 1.55 MB)
07-001 Nuc Trans, primary T cells 2007 (Adobe PDF, 1.16 MB)
07-002 Apoptosis and Mitosis 2007 (Adobe PDF, 2.97 MB)
06-001 Apoptosis, Nuclear Fragmentation 2006 (Adobe PDF, 156 KB)
06-002-1-apn Sub-cell Organelle Trafficking, ADC (Adobe PDF, 738 KB)
05-002 Shape Change, Podo Psuedopod 2005 (Adobe PDF, 1.46 MB)
05-003 Nuc Trans, Trypanosome GFP 2005 (Adobe PDF, 465 KB)
05-004 Nuc Trans, NFkB + Caspase 2005 (Adobe PDF, 1.12 MB)
05-005 Apoptosis, TUNEL False Pos 2005 (Adobe PDF, 1.66 MB)
04-001 Co-localization, Rituximab-C3b 2004 (Adobe PDF, 459 KB)

Selected Publications

Selected Publications (Adobe PDF, 221 KB)
γc deficiency precludes CD8+ T cell memory despite formation of potent T cell effectors
Decaluwe H, Taillardet M, Corcuff E, Munitic I, Law HKW, Rocha B, Riviere Y, Di Santo JP
PNAS (2010) May 3 [Epub ahead of print]

CCR9 mediates PI3K/AKT-dependent antiapoptotic signals in prostate cancer cells and inhibition of CCR9-CCL25 interaction enhances the cytotoxic effects of etoposide
Sharma PK, Singh R, Novakovic KR, Eaton JW, Grizzle WE, Singh S
Int J Cancer (2010) Feb 2 [Epub ahead of print]

Dendritic cells control T cell tonic signaling required for responsiveness to foreign antigen
Hochweller K, Wabnitz GH, Samstag Y, Suffner J, Hämmerling GJ, Garbi N.
PNAS (2010) 107(13):5931-6.

Characterization of the Murine SIRT3 Mitochondrial Localization Sequence and Comparison of Mitochondrial Enrichment and Deacetylase Activity of Long and Short SIRT3 Isoforms
Bao, J., Lu, Z., Joseph, J.J., Carabenciov, D., Dimond, C.C., Pang, L., Samsel, L., McCoy, J.P., Leclerc, J., Nguyen, P., Gius, D., and Sack, M.N.
Journal of Cellular Biochemistry (2010) 9999:1-10

Unique biological properties of catalytic domain directed human anti-CAIX antibodies discovered through phage-display technology
Xu C, Lo A, Yammanuru A, Tallarico AS, Brady K, Murakami A, Barteneva N, Zhu Q, Marasco WA.
PLoS One. 2010 Mar 10;5(3):e9625

Members of the Entamoeba histolytica transmembrane kinase family play non-redundant roles in growth and phagocytosis
Buss, S.N., Hamano, S., Vidrich, A., Evans, C., Zhang, Y., Crasta, O.R., Sobral, B.W., Gilchrist, C.A., Petri Jr., W.A.
International Journal for Parasitology (2010) Jan 18,( Epub ahead of print)

TGF-beta regulates T-cell neurokinin-1 receptor internalization and function
Beinborn, M., Blum, A., Hang, L., Setiawan, T., Schroeder, J.C., Stoyanoff, K., Leung, J., and Weinstock, J.V.
Proc Natl Acad Sci. (2010) Feb. 16 [Epub ahead of print]

HIV-1 Nef inhibits ruffles, induces filopodia and modulates migration of infected lymphocytes
Nobile, C., Rudnicka, D., Hasan, M., Aulner, N., Porrot, F., Machu, C., Renaud, O., Prevost, M.C., Hivroz, C., Schwartz, O., Sol-Foulon, N.
Journal of Virology (2009) Dec. 16 [Epub ahead of print]

Peroxiredoxin 1 Stimulates Secretion of Proinflammatory Cytokines by Binding to TLR4
Riddell, J.R., Wang, X., Minderman, H., O.Gollnick, S.
Journal of Immunology (2009) 184: 1022-30

Immune synapse formation determines interaction forces between T cells and antigen-presenting cells measured by atomic force microscopy
Hosseini, B. H., Louban, I., Djandji, D., Wabnitz, G. H., Deeg, J., Bulbuc, N., Samstag, Y., Gunzer, M., Spatz, J.,P.,Hammerling, G. J.
Proc Natl Acad Sci. (2009)106(42):17852-7. Epub 2009 Oct 12.

Numbers matter: quantitative and dynamic analysis of the formation of an immunological synapse using imaging flow cytometry
Fariyal Ahmed, Sherree Friend, Thaddeus George, Natasha Barteneva, and Judy Leiberman
Journal of Immunological Methods (2009) 125(10):2288-95

Clinical and biological significance of CXCR5 expressed by prostate cancer specimens and cell lines
Singh S, Singh R, Singh UP, Rai SN, Novakovic KR, Chung LW, Didier PJ, Grizzle WE, Lillard JW Jr.
International Journal of Cancer (2009) 125(10):2288-95

Statins inhibit NK-cell cytotoxicity by interfering with LFA-1-mediated conjugate formation
Patrick C. Raemer, Kristine Kohl and Carsten Watzl
European Journal of Immunology (2009) 39(6):1456-65

Retinoic acid induces nuclear accumulation of Raf1 during differentiation of HL-60 cells
James Smith, Rodica P. Bunaciu, Gudrun Reiterer, David Coder, Thaddeus George, Michael Asaly, Andrew Yen
Experimental Cell Research (2009)315(13):2241-48

Alpha6beta4 integrin crosslinking induces EGFR clustering and promotes EGF-mediated Rho activation in breast cancer
Michael Z Gilcrease, Xiao Zhou, Xiaolin Lu, Wendy A Woodward, Brian E Hall, and Phillip J Morrissey
Journal of Experimental & Clinical Cancer Research (2009) 28:67-77

The Urokinase Plasminogen Activator Receptor Promotes Efferocytosis of Apoptotic Cells
Veera D’mello, Sukhwinder Singh, Yi Wu, and Raymond B. Birge
The Journal of Biological Chemistry (2009) 284(25):17030–17038

NF-E2-related factor 2 regulates the stress response to UVA-1-oxidized phospholipids in skin cells
Florian Gruber, Herbert Mayer, Barbara Lengauer, Veronika Mlitz, John M. Sanders, Alexandra Kadl, Martin Bilban, Rainer de Martin Oswald Wagner, Thomas W. Kensler, Masayuki Yamamoto, Norbert Leitinger, and Erwin Tschachler
The FASEB Journal (Ahead of Print) Published online August 31, 2009

Differential Association of Programmed Death-1 and CD57 with Ex Vivo Survival of CD8⁺ T Cells in HIV Infection
Constantinos Petrovas, Benjamin Chaon, David R. Ambrozak, David A. Price, J. Joseph Melenhorst, Brenna J. Hill, Christof Geldmacher, Joseph P. Casazza,
Pratip K. Chattopadhyay, Mario Roederer, Daniel C. Douek, Yvonne M. Mueller, Jeffrey M. Jacobson, Viraj Kulkarni, Barbara K. Felber, George N. Pavlakis, Peter D. Katsikis, and Richard A. Koup
The Journal of Immunology, (2009) 183(2):1120-32

Binding of Submaximal C1q Promotes Complement-Dependent Cytotoxicity (CDC) of B Cells Opsonized with Anti-CD20 mAbs Ofatumumab (OFA) or Rituximab (RTX): Considerably Higher Levels of CDC Are Induced by OFA than by RTX
Andrew W. Pawluczkowycz, Frank J. Beurskens, Paul V. Beum, Margaret A. Lindorfer, Jan G. J. van de Winkel, Paul W. H. I. Parren, and Ronald P. Taylor
The Journal of Immunology, (2009) 183: 749–758

Identification of a dendritic cell receptor that couples sensing of necrosis to immunity
David Sancho, Olivier P. Joffre, Anna M. Keller, Neil C. Rogers, Dolores Martínez, Patricia Hernanz-Falcón, Ian Rosewell & Caetano Reis e Sousa
Nature  (2009) 458, 899-903

Distinct Regulation of Integrin-Dependent T Cell Conjugate Formation and NF-κB Activation by the Adapter Protein ADAP
Brandon J. Burbach, Rupa Srivastava, Ricardo B. Medeiros, William E. O'Gorman, Erik J. Peterson and Yoji Shimizu
The Journal of Immunology (2008) 181: 4840-4851

Binding, Internalization, and Antigen Presentation of Vaccine-Loaded Nanoengineered Capsules in Blood
Robert De Rose, Alexander N. Zelikin, Angus P. R. Johnston, Amy Sexton, Siow-Feng Chong, Christina Cortez, William Mulholland, Frank Caruso, Stephen J. Kent
Advanced Materials (2008) 20:4698-4703

Akt inhibition promotes autophagy and sensitizes PTEN-null tumors to lysosomotropic agentsMichael Degtyarev, Ann De Mazière, Christine Orr, Jie Lin, Brian B. Lee, Janet Y. Tien, Wei W. Prior, Suzanne van Dijk, Hong Wu, Daniel C. Gray, David P. Davis, Howard M. Stern, Lesley J. Murray, Klaus P. Hoeflich, Judith Klumperman, Lori S. Friedman, and Kui Lin
The Journal of Cell Biolog (2008) Vol. 183, No. 1, 101-116

Interferon regulatory factor 7-mediated responses are defective in cord blood plasmacytoid dendritic cells
Danis, B., George, T. C., Goriely, S., Dutta, B., Renneson, J., Gatto, L., Fitzgerald-Bocarsly, P., Marchant, A., Goldman, M., Willems, F., DeWit, D.
European Journal of Immunology (2008) Vol. 38, No 2, 507-17

CD44 Deficiency Attenuates Chronic Murine Ileitis
Colm B. Collins, et. al.
Gastroenterology (2008) 135:1993-2002

Lytic Granule Loading of CD8+ T Cells Is Required for HIV-Infected Cell Elimination Associated with Immune Control
Stephen A. Migueles, et. al.
Immunity (2008) 29:1009-1021

Binding of Rituximab, Trastuzumab, Cetuximab, or mAb T101 to Cancer Cells Promotes Trogocytosis Mediated by THP-1 Cells and Monocytes
Paul V. Beum, David A. Mack, Andrew W. Pawluczkowycz, Margaret A. Lindorfer, and Ronald P. Taylor
The Journal of Immunology (2008) 181(11):8120-8132

Deregulation of c-Myc Confers Distinct Survival Requirements for Memory B Cells, Plasma Cells, and Their Progenitors
Sefat E. Khuda, William M. Loo, Siegfried Janz, Brian Van Ness, and Loren D. Erickson=
The Journal of Immunology (2008) 181(11):7537-7549

Multispectral imaging of hematopoietic cells: Where flow meets morphology
Kathleen E. McGrath, Timothy P. Bushnell, James Palis
Journal of Immunological Methods (2008) 336:91-97

Quantitative image based apoptotic index measurement using multispectral imaging flow cytometry: a comparison with standard photometric methods
Shannon Henery, Thaddeus George, Brian Hall, David Basiji, William Ortyn, Philip Morrissey
Apoptosis (2008) 13:1054-1063

Optimization of Yeast Cell Cycle Analysis and Morphological Characterization by Multispectral Imaging Flow Cytometry
Meredith E. K. Calvert, Joanne A. Lannigan, Lucy F. Pemberton
Cytometry Part A (2008) 73A:825-833

Autophosphorylation docking site Tyr867 in Mertk allows for dissociation of multiple signaling pathways for phagocytosis of apoptotic cells and downmodulation of LPS-inducible NF-κB transcriptional activation
Nitu Tibrewal, Yi Wu, Veera D’mello, Reiko Akakura, Thaddeus C. George, Brian Varnum, Raymond B. Birge
Journal of Biological Chemistry (2008) 283(6):3618-3627

Image-Based Study of Interferongenic Interactions between Plasmacytoid Dendritic Cells and HSV-Infected Monocyte-Derived Dendritic Cells
Nicholas J. Megjugorac, Evan S. Jacobs, Alexander G. Izaguirre, Thaddeus C. George, Gunjan Gupta, Patricia Fitzgerald-Bocarsly
Immunological Investigations (2007) 36:739–761

Enucleation of primitive erythroid cells generates a transient population of “pyrenocytes” in the mammalian fetus
Kathleen E. McGrath, Paul D. Kingsley, Anne D. Koniski, Rebecca L. Porter, Timothy P. Bushnell, James Palis
Blood (2008) 111:2409-2417.

Morphological characterization of very small embryonic-like stem cells (VSELs) by ImageStream system analysis
Ewa K. Zuba-Surma, Magdalena Kucia, Ahmed Abdel-Latif, Buddhadeb Dawn, Brian Hall, Rajesh Singh, James W. Lillard, Jr., Mariusz Z. Ratajczak
Journal of Cellular and Molecular Medicine (2007) 12(1):292-303

IFN-α Is Not Sufficient to Drive Th1 Development Due to Lack of Stable T-bet Expression
Hilario J. Ramos, Ann M. Davis, Thaddeus C. George, J. David Farrar.
The Journal of Immunology (2007) 179:3792–3803

Cellular Image Analysis and Imaging by Flow Cytometry
David A. Basiji, William E. Ortyn, Luchuan Liang, Vidya Venkatachalam and Philip Morrissey
Clinics in Laboratory Medicine (2007) 27:653-670

Temporal Dissection of T-bet Functions
Jennifer L. Matsuda, Thaddeus C. George, James Hagman, Laurent Gapin
The Journal of Immunology (2007) 178:3457-3465

Intracellular Kaposi’s Sarcoma-Associated Herpesvirus Load Determines Early Loss of Immune Synapse Components
Laura A. Adang, Costin Tomescu, Wai K. Law, Dean H. Kedes
Journal of Virology (2007) 81(10):5079-5090

Extended Depth of Field Imaging for High Speed Cell Analysis
William E. Ortyn, David J. Perry, Vidya Venkatachalam, Luchuan Liang, Brian E. Hall, Keith Frost, David A. Basiji
Cytometry Part A (2007) 71A:215-231

Autophagy-Dependent Viral Recognition by Plasmacytoid Dendritic Cells
Heung Kyu Lee, Jennifer M. Lund, Balaji Ramanathan, Noboru Mizushima, Akiko Iwasaki
Science (2007) 315:1398-1401

The unexpected effect of cyclosporin A on CD56+CD16- and CD56+CD16+ natural killer cell subpopulations
Hongbo Wang, Bartosz Grzywacz, David Sukovich, Valarie McCullar, Qing Cao, Alisa B. Lee, Bruce R. Blazar, David N. Cornfield, Jeffrey S. Miller, Michael R. Verneris
Blood (2007) 110:1530-1539

Quantitative analysis of protein co-localization on B cells opsonized with rituximab and complement using the ImageStream multispectral imaging flow cytometer
Paul V. Beum, Margaret A. Lindorfer, Brian E. Hall, Thaddeus C. George, Keith Frost, Philip J. Morrissey, Ronald P. Taylor
Journal of Immunological Methods (2006) 317:90-99

Receptor Cross-Linking on Human Plasmacytoid Dendritic Cells Leads to the Regulation of IFN-α Production
Stacey L. Fanning, Thaddeus C. George, Di Feng, Steven B. Feldman, Nicholas J. Megjugorac, Alexander G. Izaguirre, and Patricia Fitzgerald-Bocarsly
The Journal of Immunology (2006) 177:5829-5839

Asynchronous Progression through the Lytic Cascade and Variations in Intracellular Viral Loads Revealed by High-Throughput Single-Cell Analysis of Kaposi’s Sarcoma-Associated Herpesvirus Infection
Laura A. Adang, Christopher H. Parsons, Dean H. Kedes
Journal of Virology (2006) 80:10073-10082

Sensitivity Measurement and Compensation in Spectral Imaging
William E. Ortyn, Brian E. Hall, Thaddeus C. George, Keith Frost, David A. Basiji, David J. Perry, Cathleen A. Zimmerman, David Coder, Philip J. Morrissey
Cytometry Part A (2006) 69A:852-862

Quantitative Measurement of Nuclear Translocation Events using Similarity Analysis of Multispectral Cellular Images Obtained in Flow
Thaddeus C. George, Stacey L. Fanning, Patricia Fitzgerald-Bocarsly, Ricardo B. Medeiros, Sarah Highfill, Yoji Shimizu, Brian E. Hall, Keith Frost, David A.Basiji, William E. Ortyn, Philip J. Morrissey, David H. Lynch
Journal of Immunological Methods (2006) 311:117-129

Posters

Role of PI3K and autophagy in virus-stimulated IFN-α production by human plasmacytoid dendritic cells AAI 2010 (Adobe PDF, 875 KB)
Immune Modulation Enhances the Macrophage-Mediated Clearance of Pneumocystis AAI 2010 (Adobe PDF, 573 KB)
Secreted Peroxiredoxin 1 and Toll-Like Receptor 4 Interaction mediates Immune Regulation AAI 2010 (Adobe PDF, 845 KB)
Measurement of rare innate immune cell activation directly in whole blood using imaging flow cytometry CYTO 2010 (Adobe PDF, 742 KB)
Quantitative analysis of immune synapse formation using imaging flow cytometry CYTO 2010 (Adobe PDF, 701 KB)
Automated Classification And Population Determination In Cellular Image Data CYTO 2010 (Adobe PDF, 894 KB)
Ensuring Data Quality for Image-based Cell Analysis CYTO 2010 (Adobe PDF, 1.12 MB)
Classification of Interphase and Mitotic Phases of the Cell Cycle Using Image Cytometry CYTO 2010 (Adobe PDF, 921 KB)
Characterization of mitochondrial morphology and activity in Kindler Sydrome fibroblasts CYTO 2010 (Adobe PDF, 585 KB)
SapC-DOPS Agents in Imaging Arthritis CYTO 2010 (Adobe PDF, 515 KB)
Quantitation of granzyme B granules in murine CD8+ T cells CYTO 2010 (Adobe PDF, 187 KB)
Thy-1+ Human Orbital Fibroblasts Produce a Novel Anti-adipogenic Factor  ARVO 2010 (Adobe PDF, 2.45 MB)
Kaposi’s Sarcoma-associated Herpesvirus Selectively Infects a Subset of Tonsillar B Cells   ECCMID 2010 (Adobe PDF, 1.95 MB)
C1q deficiency leads to the defective suppression of IFN-a in response to nucleoprotein containing immune complexes Midwinter Conference 2010 (Adobe PDF, 471 KB)
Acutely HIVIIIB- Infected CD4+ T Cells and Chronically-Infected HIVIIIB-H9 Cells Preferentially Conjugate and Subsequently Fuse With Plasmacytoid Dendritic Cells CCIHD 2009 (Adobe PDF, 1.23 MB)
Detection of Numerical Chromosome Aberrations in Rare Acute Myeloid Leukemia (AML) Cells EHA 2009 (Adobe PDF, 1.09 MB)
Intoxication of Non-CD11b/CD18-Expressing Cells by the Adenylate Cyclase Toxin of Bordetella pertussis ASM 2009 (Adobe PDF, 313 KB)
Increased body temperature induces lymphocyte lipid raft aggregation AAI 2009 (Adobe PDF, 1.49 MB)
T regulatory and Prostate cancer cell-specific drug targeting using novel XPclad© nanoparticle AAI 2009 (Adobe PDF, 1 MB)
Acutely HIVIIIB- Infected CD4+ T Cells and Chronically-Infected HIVIIIB-H9 Cells Preferentially Conjugate and Subsequently Fuse With Plasmacytoid Dendritic Cells AAI 2009 (Adobe PDF, 407 KB)
SHP-1 influences resistance of CD4+ T cells to regulatory T cell suppression AAI 2009 (Adobe PDF, 375 KB)
Tyrosine phosphatase SHP-1 negatively regulates suppressive potential of CD4+CD25+Foxp3+ regulatory T cells AAI 2009 (Adobe PDF, 367 KB)
Distinct regulatory roles of NF-κB in CpGA and CpGB- induced IFN-α production by plasmacytoid dendritic cells AAI 2009 (Adobe PDF, 345 KB)
PI3K and potentially autophagy are involved in HSV, Flu, SeV, and CpG A -stimulated IFN-α production by human plasmacytoid dendritic cells AAI 2009 (Adobe PDF, 846 KB)
Numbers Matters: Quantitative and Dynamic Analysis of the Formation of an Immunological Synapse using Imaging Flow Cytometry AAI 2009 (Adobe PDF, 518 KB)
Quantitative Assessment of NF-κB Activation in Heterogeneous Cell Populations AACR 2009 (Adobe PDF, 587 KB)
A novel method to assess the level of in vivo immunosuppression in transplant patients using Amnis ImageStream technology AAI 2009 (Adobe PDF, 379 KB)
Optimization of Isolation and Further Molecular and Functional Characterization of SSEA-4+/Oct-4+/CD133+/CXCR4+/Linneg/CD45neg Very Small Embryonic-Like (VSEL) Stem Cells Isolated from Umbilical Cord Blood ASH 2008 (Adobe PDF, 98 KB)
Oceanography on the ImageStream Imaging Flow Cytometer: automated quantitative image analysis of plankton, diatoms, and more October 2008 (Adobe PDF, 1.1 MB)
Quantitative measurement of apoptotic and mitotic nuclear events using automated classification of brightfield, darkfield, and nuclear fluorescent imagery with the ImageStream imaging flow cytometer AAI 2008 (Adobe PDF, 786 KB)
Automated classification of cellular images obtained on the ImageStream imaging flow cytometer ISAC 2008 (Adobe PDF, 431 KB)
Recent modifications to the ImageStream® technology improving its applicability to the study of NF-κB activation in a clinical research setting IMAT 2008 (Adobe PDF, 398 KB)
Intracellular localization and trafficking using the ImageStream® imaging flow cytometer AAI 2007 (Adobe PDF, 203 KB)
Measurement of nuclear translocation in primary T cells using the ImageStream multispectral imaging flow cytometer AAI 2007 (Adobe PDF, 1.46 MB)
Automated quantitation of events with protein localized to the T:APC synapse using the ImageStream® imaging flow cytometer  AAI 2007 (Adobe PDF, 648 KB)
Simultaneous Measurement of Apoptosis and Nuclear Translocation Events using the ImageStream® Imaging Flow Cytometer ISAC  ISAC 2006 (Adobe PDF, 597 KB)
Image Data Exploration and Analysis Software  ISAC 2006 (Adobe PDF, 482 KB)
Automated Classification of Apoptosis and Artifact Rejection in TUNEL Positive Cells ISAC 2006 (Adobe PDF, 734 KB)
Quantitative Analysis of Therapeutic Monoclonal Antibody Localizations to Endosomes and Lysosomes Using the ImageStream® Imaging Flow Cytometer AAI 2006 (Adobe PDF, 170 KB)
Kinetics of CpG internalization and sub-cellular organelle co-localization within circulating human plasmacytoid dendritic cells as measured by the ImageStream™ imaging flow cytometer ISAC 2006 (Adobe PDF, 613 KB)
Assessment of Protein Co-localization with an Imaging Flow Cytometer and Novel Analytical Method  AAI 2006 (Adobe PDF, 211 KB)


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